Host Institution : Prof. Dr. Christian Haering, European Molecular Biology Laboratory
Duration of stay: Decenber 2-11, 2018
- To build a network with Christian Hearing laboratory in which condensin proteins have been successfully purified
To learn how to purify the proteins
To lay a foundation for our condensin research.
Motivation for AI DA application
Elucidating the molecular mechanism of the condensin translocation.
In the previous study, I elucidated that condensin is a molecular motor protein that unidirectionally translocates along DNA using ATP hydrolysis energy. However, the molecular mechanism of the translocation has remained unclear. Thus, I applied for AIDA to build an international research network with the Christian Hearing laboratory, to purify the mutant condensin proteins, and to elucidate the molecular mechanism of the translocation.
I could lay a foundation for our condensin research in Kyoto.
I learned how to purify condensin proteins and purified wild-type condensin proteins in EMBL. I transported the purified proteins to Kyoto University to check their activity with fluorescent microscopy. Also, using the international research network built in this visit, I transported the plasmids and the yeast strains that are required to purify the condensin proteins from now on. Besides, I learned how to mutate the 10 kilo base pairs long plasmids and lay a foundation for our condensin research.
European Molecular Biology Laboratory
Networking with EMBL researchers, including graduates from Kyoto University.
Based on the research network I built in Germany, I would like to mutate the plasmids, purify the condensin mutants, and check their activity with fluorescent microscopy in Kyoto University. Also, I would like to teach how to purify the condensin proteins to the students in our laboratory so that they can play an important role in the condensin research. Through these activities, I would like to elucidate the molecular mechanism of the condensin translocation in future.