Activities and Researcher's Reports
Building Network for Elucidation of stepping mechanism of molecular motor Condensin
Tsuyoshi Terakawa
Assistant Professor, Graduate School of Science
Host Institution: European Molecular Biology Laboratory
Duration of stay: December 2-11, 2018

Host Institution : Prof. Dr. Christian Haering, European Molecular Biology Laboratory

Duration of stay: Decenber 2-11, 2018

Aims:

  • To build a network with Christian Hearing laboratory in which condensin proteins have been successfully purified
  • To  learn how to purify the proteins

  • To lay a foundation for our condensin research.

 

Motivation for AI DA application

Elucidating the molecular mechanism of the condensin translocation.

In the previous study, I elucidated that condensin is a molecular motor protein that unidirectionally translocates along DNA using ATP hydrolysis energy. However, the molecular mechanism of the translocation has remained unclear. Thus, I applied for AIDA to build an international research network with the Christian Hearing laboratory, to purify the mutant condensin proteins, and to elucidate the molecular mechanism of the translocation.

Results

 I could lay a foundation for our condensin research in Kyoto.

I learned how to purify condensin proteins and purified wild-type condensin proteins in EMBL. I transported the purified proteins to Kyoto University to check their activity with fluorescent microscopy. Also, using the international research network built in this visit, I transported the plasmids and the yeast strains that are required to purify the condensin proteins from now on. Besides, I learned how to mutate the 10 kilo base pairs long plasmids and lay a foundation for our condensin research.

 


European Molecular Biology Laboratory

Networking with EMBL researchers, including graduates from Kyoto University.

Outlook

Based on the research network I built in Germany, I would like to mutate the plasmids, purify the condensin mutants, and check their activity with fluorescent microscopy in Kyoto University. Also, I would like to teach how to purify the condensin proteins to the students in our laboratory so that they can play an important role in the condensin research. Through these activities, I would like to elucidate the molecular mechanism of the condensin translocation in future.